THE ACTION OF HYPOCHLOROUS ACID ON PHOSPHATIDYLCHOLINE LIPOSOMES IN DEPENDENCE ON THE CONTENT OF DOUBLE-BONDS - STOICHIOMETRY AND NMR ANALYSIS

被引：73

作者：

ARNHOLD, J ^{[1
]}

PANASENKO, OM ^{[1
]}

SCHILLER, J ^{[1
]}

VLADIMIROV, YA ^{[1
]}

ARNOLD, K ^{[1
]}

机构：

[1] RES INST PHYSICOCHEM MED, MOSCOW 119828, RUSSIA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1995年 / 78卷 / 01期

关键词：

HYPOCHLOROUS ACID; CHLOROHYDRINS; DOUBLE BONDS; PHOSPHOLIPIDS; FATTY ACIDS;

D O I：

10.1016/0009-3084(95)02484-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetics of the consumption of hypochlorous acid in its reaction with double bonds of unsaturated phospholipids and fatty acids were measured using luminol chemiluminescence. Stoichiometry ratios between the consumption of HOCl/OCl- and the loss of double bonds vary from 2:1 to 1:1. Highest values were found in DMPC liposomes containing 5 mol% oleic acid or OPPC. With increasing content of double bonds or higher numbers of double bonds in a fatty acid acyl chain due to incorporated unsaturated fatty acids or phospholipids in DMPC liposomes the stoichiometry ratio falls continuously to 1:1. A ratio of about 1:1 was observed in multilamellar and unilamellar liposomes composed of egg yolk phosphatidylcholine. Products of the reaction of oleic acid with hypochlorous acid were analysed by H-1-NMR spectroscopy. Chlorohydrins were formed in both DMPC liposomes containing 5 or 40 mol% oleic acid.

引用

页码：55 / 64

页数：10

共 37 条

[1] INACTIVATION OF THYMIDYLATE SYNTHASE BY CHLORINE DISINFECTANTS [J].