PROTEIN TRAFFIC BETWEEN DISTINCT PLASMA-MEMBRANE DOMAINS - ISOLATION AND CHARACTERIZATION OF VESICULAR CARRIERS INVOLVED IN TRANSCYTOSIS

被引：85

作者：

SZTUL, E

KAPLIN, A

SAUCAN, L

PALADE, G

机构：

[1] JOHNS HOPKINS UNIV,BALTIMORE,MD 21205

[2] UNIV CALIF SAN DIEGO,SCH MED,LA JOLLA,CA 92093

来源：

CELL | 1991年 / 64卷 / 01期

关键词：

D O I：

10.1016/0092-8674(91)90210-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi elements, basolateral plasma membrane, as well as early endosomal components. The fraction was analyzed by biochemical and immunological procedures. Antibodies raised against the proteins in the fraction recognized a single 108K antigen. Based on its subcellular distribution, the 108K antigen may represent a novel marker for transcytotic vesicular carriers.

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页码：81 / 89

页数：9

相关论文

共 47 条

[1] EVIDENCE FOR THE SORTING OF ENDOCYTIC VESICLE CONTENTS DURING THE RECEPTOR-MEDIATED TRANSPORT OF IGG ACROSS THE NEWBORN RAT INTESTINE [J].

ABRAHAMSON, DR ;

RODEWALD, R .

JOURNAL OF CELL BIOLOGY, 1981, 91 (01) :270-280

[2] PLASMA-MEMBRANE PROTEIN SORTING IN EPITHELIAL-CELLS - DO SECRETORY PATHWAYS HOLD THE KEY [J].

BARTLES, JR ;

HUBBARD, AL .

TRENDS IN BIOCHEMICAL SCIENCES, 1988, 13 (05) :181-184

[3] BIOGENESIS OF THE RAT HEPATOCYTE PLASMA-MEMBRANE INVIVO - COMPARISON OF THE PATHWAYS TAKEN BY APICAL AND BASOLATERAL PROTEINS USING SUBCELLULAR FRACTIONATION [J].

BARTLES, JR ;

FERACCI, HM ;

STIEGER, B ;

HUBBARD, AL .

JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1241-1251

[4]

Boyer J.L., 1986, PHYSL MEMBRANE DISOR, P609

[5] A COMMON SPECTRUM OF POLYPEPTIDES OCCURS IN SECRETION GRANULE MEMBRANES OF DIFFERENT EXOCRINE GLANDS [J].

CAMERON, RS ;

CAMERON, PL ;

CASTLE, JD .

JOURNAL OF CELL BIOLOGY, 1986, 103 (04) :1299-1313

[6] AUTORADIOGRAPHIC LOCALIZATION OF THE SITES OF UPTAKE, CELLULAR-TRANSPORT, AND CATABOLISM OF LOW-DENSITY LIPOPROTEINS IN THE LIVER OF NORMAL AND ESTROGEN-TREATED RATS [J].

CHAO, YS ;

JONES, AL ;

HRADEK, GT ;

WINDLER, EET ;

HAVEL, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (01) :597-601

[7] BIOSYNTHESIS OF INTESTINAL MICROVILLAR PROTEINS - EVIDENCE FOR AN INTRACELLULAR SORTING TAKING PLACE IN, OR SHORTLY AFTER, EXIT FROM THE GOLGI-COMPLEX [J].

DANIELSEN, EM ;

COWELL, GM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 152 (02) :493-499

[8] ISOLATION OF EXOCYTIC CARRIER VESICLES FROM BHK CELLS [J].

DECURTIS, I ;

SIMONS, K .

CELL, 1989, 58 (04) :719-727

[9] IMMUNO-ISOLATION OF A PLASMA-MEMBRANE FRACTION FROM THE FAO CELL [J].

DEVANEY, E ;

HOWELL, KE .

EMBO JOURNAL, 1985, 4 (12) :3123-3130

[10] RECEPTOR-MEDIATED ENDOCYTOSIS OF EPIDERMAL GROWTH-FACTOR BY RAT HEPATOCYTES - RECEPTOR PATHWAY [J].

DUNN, WA ;

CONNOLLY, TP ;

HUBBARD, AL .

JOURNAL OF CELL BIOLOGY, 1986, 102 (01) :24-36

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