A RAPID AND SIMPLE METHOD FOR THE MEASUREMENT OF NITRITE AND NITRATE IN PLASMA BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

被引：190

作者：

LEONE, AM ^{[1
]}

FRANCIS, PL ^{[1
]}

RHODES, P ^{[1
]}

MONCADA, S ^{[1
]}

机构：

[1] UNIV DUNDEE,NINEWELLS HOSP & MED SCH,DEPT CLIN PHARMACOL,DUNDEE DD1 9SY,SCOTLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1542

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantification of nitric oxide in vivo is difficult because of its short half-life. Instead the oxidised products, nitrite and nitrate, are commonly used as indices of nitric oxide generation. We describe here a simple method for the measurement of nitrite and nitrate in plasma using high performance capillary electrophoresis. Plasma standard curves gave regression coefficients of 0.98 for nitrite and 0.99 for nitrate with an intra-assay coefficient of variance of 4.6% for 50 mu M nitrite (n=10) and 1.2% for 50 mu M nitrate (n=10). The assay allows measurement of nitrite and nitrate in a single analysis requiring minimal sample preparation, and with the sensitivity to detect both basal and physiological changes in plasma nitrite and nitrate. (C) Academic Press, Inc.

引用

页码：951 / 957

页数：7

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