GENOTYPIC IDENTIFICATION OF MYCOBACTERIA BY NUCLEIC-ACID SEQUENCE DETERMINATION - REPORT OF A 2-YEAR EXPERIENCE IN A CLINICAL LABORATORY

被引：428

作者：

KIRSCHNER, P ^{[1
]}

SPRINGER, B ^{[1
]}

VOGEL, U ^{[1
]}

MEIER, A ^{[1
]}

WREDE, A ^{[1
]}

KIEKENBECK, M ^{[1
]}

BANGE, FC ^{[1
]}

BOTTGER, EC ^{[1
]}

机构：

[1] HANNOVER MED SCH,INST MED MIKROBIOL,KONSTANTY GUTSCHOW STR 8,D-30623 HANNOVER,GERMANY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 11期

关键词：

D O I：

10.1128/JCM.31.11.2882-2889.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.

引用

页码：2882 / 2889

页数：8

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