2-DIMENSIONAL PROTEIN MAP OF SACCHAROMYCES-CEREVISIAE - CONSTRUCTION OF A GENE-PROTEIN INDEX

被引：72

作者：

BOUCHERIE, H ^{[1
]}

DUJARDIN, G ^{[1
]}

KERMORGANT, M ^{[1
]}

MONRIBOT, C ^{[1
]}

SLONIMSKI, P ^{[1
]}

PERROT, M ^{[1
]}

机构：

[1] CNRS, CTR GENET MOLEC, F-91198 GIF SUR YVETTE, FRANCE

来源：

YEAST | 1995年 / 11卷 / 07期

关键词：

SACCHAROMYCES; YEAST PROTEIN MAP; PROTEIN IDENTIFICATION;

D O I：

10.1002/yea.320110702

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This publication marks the beginning of the construction of a gene-protein index that relates proteins which are resolved on the two-dimensional protein map of Saccharomyces cerevisiae with their corresponding genes. We report the identification of 36 novel polypeptide spots on the yeast protein map. They correspond to the products of 26 genes. Together with the polypeptide spots previously identified, this raises to 41 the number of genes whose products have been identified on the protein map. The proteins identified here are concerned with four major areas of yeast cellular physiology: carbon metabolism, heat shock, amino acid biosynthesis and purine biosynthesis. Given the molecular weight and isoelectric point of the identified proteins, and the codon-usage bias of the corresponding genes, it can be estimated that 25 to 35% of all the soluble yeast proteins are detectable under the labelling and running gel conditions used in this study.

引用

页码：601 / 613

页数：13

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