学术探索
学术期刊
新闻热点
数据分析
智能评审

TELOMERASE ACTIVITY IN NORMAL AND MALIGNANT HEMATOPOIETIC-CELLS

被引：697
作者：
BROCCOLI, D
YOUNG, JW
DELANGE, T
机构：
[1] ROCKEFELLER UNIV,CELL BIOL & GENET LAB,NEW YORK,NY 10021
[2] ROCKEFELLER UNIV,CELLULAR PHYSIOL & IMMUNOL LAB,NEW YORK,NY 10021
[3] CORNELL UNIV,COLL MED,MEM SLOAN KETTERING CANC CTR,DIV HEMATOL ONCOL,NEW YORK,NY 10021
[4] CORNELL UNIV,COLL MED,MEM SLOAN KETTERING CANC CTR,DIV HEMATOL ONCOL,CLIN IMMUNOL SERV,NEW YORK,NY 10021
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 20期
关键词：
TELOMERE DYNAMICS; CHROMOSOME STRUCTURE; CELL IMMORTALIZATION; LEUKEMIA;
D O I：
10.1073/pnas.92.20.9082
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Bone marrow and peripheral blood leukocytes from 19 leukemia patients were found to contain telomerase activity detectable by a PCR-based assay. Telomerase was also detectable in nonmalignant bone marrow and peripheral blood leukocytes from normal donors, including fractions enriched for granulocytes, T lymphocytes, and monocytes/B cells. Semiquantitative comparison revealed considerable overlap between telomerase activities in samples from normal subjects and leukemia patients, confounding evaluation of the role of telomerase in this disease, These data indicate that human telomerase is not restricted to immortal cells and suggest that the somatic expression of this enzyme may be more widespread than was previously inferred from the decline of human telomeres.
引用
收藏
页码：9082 / 9086
页数：5
相关论文
共 25 条
[1]   TELOMERIC REPEAT FROM T-THERMOPHILA CROSS HYBRIDIZES WITH HUMAN TELOMERES [J].
ALLSHIRE, RC ;
GOSDEN, JR ;
CROSS, SH ;
CRANSTON, G ;
ROUT, D ;
SUGAWARA, N ;
SZOSTAK, JW ;
FANTES, PA ;
HASTIE, ND .
NATURE, 1988, 332 (6165) :656-659
[2]   TELOMERE LENGTH PREDICTS REPLICATIVE CAPACITY OF HUMAN FIBROBLASTS [J].
ALLSOPP, RC ;
VAZIRI, H ;
PATTERSON, C ;
GOLDSTEIN, S ;
YOUNGLAI, EV ;
FUTCHER, AB ;
GREIDER, CW ;
HARLEY, CB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10114-10118
[3]  
BLACKBURN E, 1983, REVERSE TRANSCRIPTAS, P557
[4]  
COOKE HJ, 1986, COLD SPRING HARB SYM, V51, P213, DOI 10.1101/SQB.1986.051.01.026
[5]   TELOMERASE ACTIVITY IN NORMAL LEUKOCYTES AND IN HEMATOLOGIC MALIGNANCIES [J].
COUNTER, CM ;
GUPTA, J ;
HARLEY, CB ;
LEBER, B ;
BACCHETTI, S .
BLOOD, 1995, 85 (09) :2315-2320
[6]   TELOMERASE ACTIVITY IN HUMAN OVARIAN-CARCINOMA [J].
COUNTER, CM ;
HIRTE, HW ;
BACCHETTI, S ;
HARLEY, CB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :2900-2904
[7]   TELOMERE SHORTENING ASSOCIATED WITH CHROMOSOME INSTABILITY IS ARRESTED IN IMMORTAL CELLS WHICH EXPRESS TELOMERASE ACTIVITY [J].
COUNTER, CM ;
AVILION, AA ;
LEFEUVRE, CE ;
STEWART, NG ;
GREIDER, CW ;
HARLEY, CB ;
BACCHETTI, S .
EMBO JOURNAL, 1992, 11 (05) :1921-1929
[8]   STABILIZATION OF SHORT TELOMERES AND TELOMERASE ACTIVITY ACCOMPANY IMMORTALIZATION OF EPSTEIN-BARR VIRUS-TRANSFORMED HUMAN B-LYMPHOCYTES [J].
COUNTER, CM ;
BOTELHO, FM ;
WANG, P ;
HARLEY, CB ;
BACCHETTI, S .
JOURNAL OF VIROLOGY, 1994, 68 (05) :3410-3414
[9]   ACTIVATION OF TELOMERASE IN A HUMAN TUMOR [J].
DELANGE, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :2882-2885
[10]   STRUCTURE AND VARIABILITY OF HUMAN-CHROMOSOME ENDS [J].
DELANGE, T ;
SHIUE, L ;
MYERS, RM ;
COX, DR ;
NAYLOR, SL ;
KILLERY, AM ;
VARMUS, HE .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (02) :518-527
123