CATALYSIS OF ATP-DEPENDENT HOMOLOGOUS DNA PAIRING AND STRAND EXCHANGE BY YEAST RAD51 PROTEIN

被引：746

作者：

SUNG, P

机构：

[1] Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston

来源：

SCIENCE | 1994年 / 265卷 / 5176期

关键词：

D O I：

10.1126/science.8066464

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair. Here it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-stranded form. The product of synapsis between these DNA partners is further processed by RAD51 to yield nicked circular duplex DNA, which indicates that RAD51 can catalyze strand exchange. The pairing and strand exchange reaction requires adenosine triphosphate, a result consistent with the presence of a DNA-dependent adenosine triphosphatase activity in RAD51 protein. Thus, RAD51 is a eukaryotic recombination protein that can catalyze the strand exchange reaction.

引用

页码：1241 / 1243

页数：3

共 20 条

[1] SEMIDOMINANT SUPPRESSORS OF SRS2 HELICASE MUTATIONS OF SACCHAROMYCES-CEREVISIAE MAP IN THE RAD51 GENE, WHOSE SEQUENCE PREDICTS A PROTEIN WITH SIMILARITIES TO PROKARYOTIC RECA PROTEINS [J].