IDENTIFICATION AND CHARACTERIZATION OF HITHERTO UNKNOWN MYCOPLASMA-PNEUMONIAE PROTEINS

被引：56

作者：

PROFT, T ^{[1
]}

HERRMANN, R ^{[1
]}

机构：

[1] UNIV HEIDELBERG, ZMBH, D-69120 HEIDELBERG, GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 13卷 / 02期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb00427.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven hitherto unknown Mycoplasma pneumoniae proteins were identified and characterized with respect to their size and subcellular location. This was carried out through the construction of in vitro gene fusions between a modified mouse dehydrofolate reductase (dhfr) gene and selected regions (cosmid clones) of the M. pneumoniae genome and expressing them in Escherichia coli. Positive clones were identified using antibodies against specific fractions of M. pneumoniae. The deduced protein sequences of 11 out of 30 clones did not show significant homologies to known proteins in protein databank searches. Monospecific antibodies against these 11 fusion proteins were used to determine the size and cellular location of the corresponding M. pneumoniae proteins by immunoscreening Western blots of SDS-acrylamide gels from M. pneumoniae cell extracts.

引用

页码：337 / 348

页数：12

共 64 条

[1] ISOLATION OF PHOSPHOPROTEINS BY IMMOBILIZED METAL (FE-3+) AFFINITY-CHROMATOGRAPHY [J].