REMODELING SPERM CHROMATIN IN XENOPUS-LAEVIS EGG EXTRACTS - THE ROLE OF CORE HISTONE PHOSPHORYLATION AND LINKER HISTONE B4 IN CHROMATIN ASSEMBLY

被引：114

作者：

DIMITROV, S ^{[1
]}

DASSO, MC ^{[1
]}

WOLFFE, AP ^{[1
]}

机构：

[1] NICHHD,MOLEC EMBRYOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CELL BIOLOGY | 1994年 / 126卷 / 03期

关键词：

D O I：

10.1083/jcb.126.3.591

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We find that the remodeling of the condensed Xenopus laevis sperm nucleus into the paternal pronucleus in egg extracts is associated with phosphorylation of the core histones H2A, H2A.X and H4, and uptake of a linker histone B4 and a HMG 2 protein. Histone B4 is required for the assembly of chromatosome structures in the pronucleus. However neither B4 nor core histone phosphorylation are required for the assembly of spaced nucleosomal arrays. We suggest that the spacing of nucleosomal arrays is determined by interaction between adjacent histone octamers under physiological assembly conditions.

引用

页码：591 / 601

页数：11

共 89 条

[1] SYNTHESIS OF SPERM-SPECIFIC BASIC NUCLEAR PROTEINS (SPS) IN CULTURED SPERMATIDS FROM XENOPUS-LAEVIS [J].