A ROLE FOR A PERTUSSIS-TOXIN-SENSITIVE TRIMERIC G-PROTEIN IN STORE-OPERATED CA2+ INFLOW IN HEPATOCYTES

被引：22

作者：

BERVEN, LA ^{[1
]}

BARRITT, GJ ^{[1
]}

机构：

[1] FLINDERS UNIV S AUSTRALIA,SCH MED,DEPT MED BIOCHEM,ADELAIDE,SA 5001,AUSTRALIA

来源：

FEBS LETTERS | 1994年 / 346卷 / 2-3期

基金：

英国医学研究理事会;

关键词：

CA2+ INFLOW; HEPATOCYTE; TRIMERIC G-PROTEIN; PERTUSSIS TOXIN; INOSITOL TRISPHOSPHATE;

D O I：

10.1016/0014-5793(94)00481-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P-4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP(2)) and 'caged' guanosine 5'-[gamma thio]triphosphate (GTP gamma S) (introduced into the cytoplasmic space by microinjection), thapsigargin and 2,5-di-tert-butylhydroquinone (DBHQ) to stimulate Ca2+ inflow. Photolysis of 'caged' GPIP(2) or 'caged' GTP gamma S stimulated Ca2+ inflow. The abilities of GPIP(2), thapsigargin and DBHQ to stimulate Ca2+ inflow were inhibited by the pre-treatment of hepatocytes with pertussis toxin in vivo for 36 h. Thapsigargin-stimulated Ca2+ inflow was also inhibited by guanosine 5'-[beta-thio]diphosphate (GDP beta S) (introduced by microinjection). It is concluded that, in hepatocytes, store-operated Ca2+ inflow induced by the actions of either inositol 1,4,5-trisphosphate, thapsigargin or DBHQ requires a pertussis toxin-sensitive trimeric G-protein.

引用

页码：235 / 240

页数：6

共 35 条

[1] THE NATURE AND MECHANISM OF ACTIVATION OF THE HEPATOCYTE RECEPTOR-ACTIVATED CA-2+ INFLOW SYSTEM [J].

BARRITT, GJ ;

HUGHES, BP .

CELLULAR SIGNALLING, 1991, 3 (04) :283-292

[2] INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].

BERRIDGE, MJ .

NATURE, 1993, 361 (6410) :315-325

[3]

BERVEN LA, 1994, IN PRESS BIOCH J

[4]

BIRD GS, 1992, J BIOL CHEM, V267, P17722

[5]

BIRD GS, 1993, J BIOL CHEM, V268, P21486

[6] DIABETES-INDUCED ALTERATIONS IN THE EXPRESSION, FUNCTIONING AND PHOSPHORYLATION STATE OF THE INHIBITORY GUANINE-NUCLEOTIDE REGULATORY PROTEIN GI-2 IN HEPATOCYTES [J].

BUSHFIELD, M ;

GRIFFITHS, SL ;

MURPHY, GJ ;

PYNE, NJ ;

KNOWLER, JT ;

MILLIGAN, G ;

PARKER, PJ ;

MOLLNER, S ;

HOUSLAY, MD .

BIOCHEMICAL JOURNAL, 1990, 271 (02) :365-372

[7]

BUTTA N, 1993, J BIOL CHEM, V268, P6081

[8] THE LIVER-CELL PLASMA-MEMBRANE CA-2+ INFLOW SYSTEMS EXHIBIT A BROAD SPECIFICITY FOR DIVALENT METAL-IONS [J].

CROFTS, JN ;

BARRITT, GJ .

BIOCHEMICAL JOURNAL, 1990, 269 (03) :579-587

[9]

ECKSTEIN F, 1979, J BIOL CHEM, V254, P9829

[10] CA2+ AND MN2+ INFLUX THROUGH RECEPTOR-MEDIATED ACTIVATION OF NONSPECIFIC CATION CHANNELS IN MAST-CELLS [J].

FASOLATO, C ;

HOTH, M ;

MATTHEWS, G ;

PENNER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3068-3072

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