A CONSENSUS REPEAT SEQUENCE FROM THE HUMAN INSULIN GENE LINKED POLYMORPHIC REGION ADOPTS MULTIPLE QUADRIPLEX DNA STRUCTURES INVITRO

被引：30

作者：

HAMMONDKOSACK, MCU ^{[1
]}

DOCHERTY, K ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,QUEEN ELIZABETH HOSP,DEPT CHEM,BIRMINGHAM B15 2TH,W MIDLANDS,ENGLAND

来源：

FEBS LETTERS | 1992年 / 301卷 / 01期

关键词：

G4; DNA; INSULIN GENE LINKED POLYMORPHIC REGION; INSULIN GENE; DNA STRUCTURE;

D O I：

10.1016/0014-5793(92)80214-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A hypervariable region consisting of repeats of a 14 base pair (bp) consensus sequence ACAGGGGT(G/C))T/C)GGGG is located 363 bp upstream of the human insulin gene. Different repeat numbers if this oligonucleotide give rise to a polymorphism, and so this region is commonly known as the insulin gene linked polymorphic region (ILPR). Here we present evidence, based on the mobility in non-denaturing polyacrylamide gels of two dissimilarly sized oligonucleotides containing the ILPR consensus sequence, that this sequence can adopt a number of quadriplex DNA structures in vitro.

引用

页码：79 / 82

页数：4

共 8 条

[1] THE HIGHLY POLYMORPHIC REGION NEAR THE HUMAN INSULIN GENE IS COMPOSED OF SIMPLE TANDEMLY REPEATING SEQUENCES [J].

BELL, GI ;

SELBY, MJ ;

RUTTER, WJ .

NATURE, 1982, 295 (5844) :31-35

[2] STRUCTURE AND FUNCTION OF TELOMERES [J].

BLACKBURN, EH .

NATURE, 1991, 350 (6319) :569-573

[3]

CLARK AR, 1992, IN PRESS INSULIN MOL

[4] THE HUMAN INSULIN GENE LINKED POLYMORPHIC REGION EXHIBITS AN ALTERED DNA-STRUCTURE [J].

HAMMONDKOSACK, MCU ;

DOBRINSKI, B ;

LURZ, R ;

DOCHERTY, K ;

KILPATRICK, MW .

NUCLEIC ACIDS RESEARCH, 1992, 20 (02) :231-236

[5]

Sambrook J., 1989, MOL CLONING LAB MANU

[6] A SODIUM-POTASSIUM SWITCH IN THE FORMATION OF 4-STRANDED G4-DNA [J].

SEN, D ;

GILBERT, W .

NATURE, 1990, 344 (6265) :410-414

[7] The structure of telomeric DNA: DNA quadriplex formation [J].

Sen, Dipankar ;

Gilbert, Walter .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1991, 1 (03) :435-438

[8] MONO-VALENT CATION INDUCED STRUCTURE OF TELOMERIC DNA - THE G-QUARTET MODEL [J].

WILLIAMSON, JR ;

RAGHURAMAN, MK ;

CECH, TR .

CELL, 1989, 59 (05) :871-880

← 1 →