MOLECULAR-CLONING OF A CDNA-ENCODING ALDOSTERONE SYNTHASE CYTOCHROME-P-450 IN RAT ADRENAL-CORTEX

被引：100

作者：

IMAI, M ^{[1
]}

SHIMADA, H ^{[1
]}

OKADA, Y ^{[1
]}

MATSUSHIMAHIBIYA, Y ^{[1
]}

OGISHIMA, T ^{[1
]}

ISHIMURA, Y ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT BIOCHEM,SHINJUKU KU,TOKYO 160,JAPAN

来源：

FEBS LETTERS | 1990年 / 263卷 / 02期

关键词：

(Rat); Aldosterone; Complementary DNA; Cytochrome P-450; Molecular cloning; Steroid-11-hydroxylase;

D O I：

10.1016/0014-5793(90)81398-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P-450s sequenced to date, and also a portion (residues 25-44) which was identical to the N-terminal peptide sequence of rat aldosterone synthase cytochrome P-450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P-450. © 1990.

引用

页码：299 / 302

页数：4

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