NEAR-UV-INDUCED BREAKS IN PHAGE DNA - SENSITIZATION BY HYDROGEN-PEROXIDE (A TRYPTOPHAN PHOTOPRODUCT)

被引：71

作者：

ANANTHASWAMY, HN ^{[1
]}

EISENSTARK, A ^{[1
]}

机构：

[1] UNIV MISSOURI, DIV BIOL SCI, COLUMBIA, MO 65201 USA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1976年 / 24卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1976.tb06851.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Near-UV irradiation of L-tryptophan yields a large number of photoproducts. When this mixture is added to recombinationless (rec) mutants of bacteria, the cells are killed. The most toxic component of tryptophan photoproducts was identified as H2O2. In this report tryptophan photoproducts and H2O2 sensitized phage DNA [from T2, T3, T4, T5 and T7] to near-UV radiation resulting in enhanced killing and enhanced DNA breakages. The in situ production of H2O2 via tryptophan photolysis may be an important biological event.

引用

页码：439 / 442

页数：4

共 19 条

[1]

[Anonymous], 1967, METH VIROL

[2] SENSITIVITY OF SALMONELLA-TYPHIMURIUM RECOMBINATIONLESS (REC) MUTANTS TO VISIBLE AND NEAR-VISIBLE LIGHT [J].

EISENSTA.A .

MUTATION RESEARCH, 1970, 10 (01) :1-&

[3] MUTAGENIC AND LETHAL EFFECTS OF VISIBLE AND NEAR-ULTRAVIOLET LIGHT ON BACTERIAL CELLS [J].

EISENSTARK, A .

ADVANCES IN GENETICS, 1971, 16 :167-+

[4] INACTIVATING DNA ALTERATIONS INDUCED BY PEROXIDES AND PEROXIDE-PRODUCING AGENTS [J].

FREESE, EB ;

GERSON, J ;

TABER, H ;

RHAESE, HJ ;

FREESE, E .

MUTATION RESEARCH, 1967, 4 (05) :517-&

[5] MUTAGENESIS BY NEAR-VISIBLE LIGHT [J].

KUBITSCHEK, HE .

SCIENCE, 1967, 155 (3769) :1545-+

[6]

MCCORMICK JP, 1976, SCIENCE, V191, P468

[7] CHEMICAL ANALYSIS OF DNA ALTERATIONS .2. ALTERATION AND LIBERATION OF BASES OF DEOXYNUCLEOTIDES AND DEOXYNUCLEOSIDES INDUCED BY HYDROGEN PEROXIDE AND HYDROXYLAMINE [J].

RHAESE, HJ ;

FREESE, E ;

MELZER, MS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 155 (02) :491-&

[8] CHEMICAL ANALYSIS OF DNA ALTERATIONS .I. BASE LIBERATION AND BACKBONE BREAKAGE OF DNA AND OLIGODEOXYADENYLIC ACID INDUCED BY HYDROGEN PEROXIDE AND HYDROXYLAMINE [J].

RHAESE, HJ ;

FREESE, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 155 (02) :476-&

[9]

THOMAS CA, 1966, PROCEDURES NUCLEIC A, P553

[10] INDUCTION OF SINGLE-STRAND BREAKS (ALKALI-LABILE BONDS) IN BACTERIAL AND PHAGE DNA BY NEAR UV (365 NM) RADIATION [J].

TYRRELL, RM ;

LEY, RD ;

WEBB, RB .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1974, 20 (05) :395-398

← 1 2 →