PREPARATIVE INVITRO MESSENGER-RNA SYNTHESIS USING SP6 AND T7 RNA-POLYMERASES

被引：142

作者：

GUREVICH, VV ^{[1
]}

POKROVSKAYA, ID ^{[1
]}

OBUKHOVA, TA ^{[1
]}

ZOZULYA, SA ^{[1
]}

机构：

[1] MM SHEMYAKIN BIOORGAN CHEM INST,PUSHCHINO 142292,USSR

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90318-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6-12 ml, yielding 4.5-18 mg of homogeneous biologically active mRNA. © 1991.

引用

页码：207 / 213

页数：7

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