2 PATHWAYS IN THE BIOSYNTHESIS OF CADYSTINS (LAMBDA-EC)NG IN THE CELL-FREE SYSTEM OF THE FISSION YEAST

被引：59

作者：

HAYASHI, Y ^{[1
]}

NAKAGAWA, CW ^{[1
]}

MUTOH, N ^{[1
]}

ISOBE, M ^{[1
]}

GOTO, T ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH AGR,NAGOYA,AICHI 464,JAPAN

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1991年 / 69卷 / 2-3期

关键词：

CADYSTIN; FISSION YEAST; CELL-FREE BIOSYNTHESIS; DIPEPTIDYL TRANSFERASE;

D O I：

10.1139/o91-018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small metal-binding peptides, cadystins, with the general structure of (gamma-Glu-Cys)n-Gly ((gamma-EC)nG), were synthesized in a cell-free system of fission yeast to examine the in vivo synthetic pathway. The crude enzyme for cadystin synthesis was prepared by ammonium sulfate precipitation (75% saturation) from the 120 000 x g supernatant of the cell extract, and the excess salt in the enzyme fraction was removed by Sephadex gel filtration. Using this crude enzyme fraction, it was shown that there were two pathways for cadystin biosynthesis. One pathway is gamma-Glu-Cys (gamma-EC) dipeptidyl transfer from both glutathione (gamma-ECG) and cadystins to glutathione and cadystins. The other one is gamma-EC polymerization from (gamma-EC)n and glutathione to (gamma-EC)n+i, followed by glycine addition with glutathione synthetase.

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页码：115 / 121

页数：7

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