HIGH-VOLTAGE AND LOW-VOLTAGE ACTIVATED CALCIUM CURRENTS ARE EXPRESSED BY NEURONS CULTURED FROM EMBRYONIC RAT NEOSTRIATUM

Current-clamp studies have shown that voltage-dependent Ca currents are present in rat neostriatal neurons. Although these studies have provided evidence for the presence of high-voltage activated Ca channels, it has been unclear whether low-voltage activated channels are also present. Using the whole-cell variant of the patch-clamp technique, we have studied isolated Ca currents in an attempt to answer this question. We have found that both high- and low-voltage activated calcium currents are expressed by neostriatal neurons cultured from embryonic rat brain. These currents are similar in voltage-dependence and pharmacology to those found in other brain neurons.