THE RHO-FAMILY GTPASES RHOA, RAC1, AND CDC42HS REGULATE TRANSCRIPTIONAL ACTIVATION BY SRF

被引:1194
作者
HILL, CS [1 ]
WYNNE, J [1 ]
TREISMAN, R [1 ]
机构
[1] IMPERIAL CANC RES FUND,TRANSCRIPT LAB,LONDON WC2A 3PX,ENGLAND
关键词
D O I
10.1016/S0092-8674(05)80020-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The c-fos serum response element (SRE) forms a ternary complex with the transcription factors SRF (serum response factor) and TCF (ternary complex factor). By itself, SRF can mediate transcriptional activation induced by serum, lysophosphatidic acid, or intracellular activation of heterotrimeric G proteins. Activated forms of the Rho family GTPases RhoA, Rad, and CDC42Hs also activate transcription via SRF and act synergistically at the SRE with signals that activate TCF. Functional Rho is required for signaling to SRF by several stimuli, but not by activated CDC42Hs or Rac1. Activation of the SRF-linked signaling pathway does not correlate with activation of the MAP kinases ERK, SAPK/JNK, or MPK2/p38. Functional Rho is required for regulated activity of the c-fos promoter. These results establish SRF as a nuclear target of a novel Rho-mediated signaling pathway.
引用
收藏
页码:1159 / 1170
页数:12
相关论文
共 49 条
[1]  
Ammerer, Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast, Genes Dev., 4, pp. 299-312, (1990)
[2]  
Bannister, Gottlieb, Kouzarides, Jackson, c-Jun is phosphorylated by the DNA-dependent protein kinase in vitro: definition of the minimal kinase recognition motif, Nucl. Acids Res., 21, pp. 1289-1295, (1993)
[3]  
Bigay, Deterre, Pfister, Chabre, Fluoroaluminates activate transducin-GDP by mimicking the γ-phosphate of GTP in its binding site, FEBS Lett., 191, pp. 181-185, (1985)
[4]  
Boguski, McCormick, Proteins regulating Ras and its relatives, Nature, 366, pp. 643-654, (1993)
[5]  
Bruhn, Sprague, MCM1 point mutants deficient in expression of α-specific genes: residues important for interaction with α1, Mol. Cell. Biol., 14, pp. 2534-2544, (1994)
[6]  
Chant, Stowers, GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more, Cell, 81, pp. 1-4, (1995)
[7]  
Chong, Traynor, Bokoch, Schwartz, The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells, Cell, 79, pp. 507-513, (1994)
[8]  
Cooper, Straight and narrow or tortuous and intersecting?, Curr. Biol., 4, pp. 1118-1121, (1994)
[9]  
Coso, Chiariello, Kalinec, Kyriakis, Woodgett, Gutkind, Transforming G-protein-coupled receptors potently activate jnk (sapk): evidence for a divergence from the tyrosine kinase signaling pathway, J. Biol. Chem., 270, pp. 5620-5624, (1995)
[10]  
Coso, Chiariello, Yu, Teramoto, Crespo, Xu, Miki, Gutkind, The Small GTP-Binding Proteins Rac1 and Cdc42 Regulate the Activity of the JNK/SAPK Signaling Pathways, Cell, 81, (1995)