THE BIOACTIVE PHOSPHOLIPID LYSOPHOSPHATIDIC ACID IS RELEASED FROM ACTIVATED PLATELETS

被引：602

作者：

EICHHOLTZ, T ^{[1
]}

JALINK, K ^{[1
]}

FAHRENFORT, I ^{[1
]}

MOOLENAAR, WH ^{[1
]}

机构：

[1] NETHERLANDS CANC INST,DIV CELLULAR BIOCHEM,PLESMANLAAN 121,1066 CX AMSTERDAM,NETHERLANDS

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

关键词：

D O I：

10.1042/bj2910677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysophosphatidic acid (LPA) is a water-soluble phospholipid with hormone-like and growth-factor-like activities. LPA activates a putative G-protein-coupled receptor in responsive cells, but the natural source of exogenous LPA is unknown. Here we show that LPA is present in mammalian serum in an active form (bound to albumin) at concentrations of 1-5 muM, but is not detectable in platelet-poor plasma, suggesting that LPA is produced during blood clotting. We find that thrombin activation of platelets prelabelled with [P-32]P(i) results in the rapid release of newly formed [P-32]LPA into the extracellular environment. We conclude that LPA is a novel platelet-derived lipid mediator that may play a role in inflammatory and proliferative responses to injury.

引用

页码：677 / 680

页数：4

共 36 条

[1]

AMES BN, 1960, J BIOL CHEM, V235, P769

[2]

BENTON AM, 1982, BLOOD, V60, P642

[3]

BILLAH MM, 1981, J BIOL CHEM, V256, P5399

[4]

BISHOP WR, 1988, ANNU REV CELL BIOL, V4, P579, DOI 10.1146/annurev.cb.04.110188.003051

[5] SELECTIVE LOSS OF LYSOPHOSPHOLIPIDS IN SOME COMMONLY USED LIPID-EXTRACTION PROCEDURES [J].

BJERVE, KS ;

DAAE, LNW ;

BREMER, J .

ANALYTICAL BIOCHEMISTRY, 1974, 58 (01) :238-245

[6] PATHWAYS OF FATTY ACID METABOLISM IN HUMAN PLATELETS [J].

COHEN, P ;

DERKSEN, A ;

BOSCH, HVD .

JOURNAL OF CLINICAL INVESTIGATION, 1970, 49 (01) :128-&

[7]

DAS AK, 1992, J BIOL CHEM, V267, P9724

[8]

DURIEUX ME, 1992, AM J PHYSIOL, V263, P896

[9] PHOSPHATIDIC-ACID AND LYSOPHOSPHATIDIC ACID STIMULATE RECEPTOR-REGULATED MEMBRANE CURRENTS IN THE XENOPUS-LAEVIS OOCYTE [J].

FERGUSON, JE ;

HANLEY, MR .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 297 (02) :388-392

[10] LYSOPHOSPHATIDIC ACID INDUCES INWARD CURRENTS IN XENOPUS-LAEVIS OOCYTES - EVIDENCE FOR AN EXTRACELLULAR SITE OF ACTION [J].

FERNHOUT, BJH ;

DIJCKS, FA ;

MOOLENAAR, WH ;

RUIGT, GSF .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1992, 213 (02) :313-315

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