FORMATION OF 5-FORMYLURACIL BY CELL-FREE PREPARATIONS FROM NEUROSPORA CRASSA

被引：26

作者：

ABBOTT, MT

DRAGILA, TA

MCCROSKE.RP

机构：

[1] Department of Chemistry, San Diego State College, San Diego, CA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 169卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2787(68)90002-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A cell-free preparation has been obtained from Neurospora crassa which catalyzes the conversion of 5-hydroxymethyluracil to 5-formyluracil. 2. This enzymatic activity was markedly stimulated by Fe(II), α-ketoglutarate, and ascorbate. 3. While the requirements for Fe(II) and α-ketoglutarate appeared quite stringent, several common biological reducing agents, e.g., GSH, substituted for ascorbate. 4. The lack of activity observed when the incubations were carried out under nitrogen suggested that oxygen is required. © 1968.

引用

页码：1 / &

共 15 条

[1] COFACTOR REQUIREMENTS OF THYMINE 7-HYDROXYLASE [J].