DEAMIDATION OF ASPARAGINE AND GLUTAMINE RESIDUES IN PROTEINS AND PEPTIDES - STRUCTURAL DETERMINANTS AND ANALYTICAL METHODOLOGY

Non-enzymatic deamidation of asparagine and glutamine residues in proteins and peptides are reviewed by first outlining the well-described reaction mechanism involving cyclic imide intermediates, followed by a discussion of structural features which influence the reaction rate. The second and major part describes analytical techniques that allow studying deamidation in proteins using recombinant human growth hormone and recombinant hirudin as examples. Finally, the significance of non-enzymatic deamidation with respect to the production of pharmaceutical proteins is discussed.