NITRIC-OXIDE RELEASE IS PRESENT FROM INCUBATED SKELETAL-MUSCLE PREPARATIONS

被引:357
作者
BALON, TW
NADLER, JL
机构
[1] DDEM, City of Hope National Medical Center, Duarte
关键词
ENDOTHELIUM-DERIVED RELAXING FACTOR; ELECTRICAL STIMULATION; EXERCISE; EXTENSOR DIGITORUM LONGUS; ISOLATED MUSCLE INCUBATION; 2-DEOXYGLUCOSE TRANSPORT; L-ARGININE; N-G-MONOMETHYL-L-ARGININE; RAT; SODIUM NITROPRUSSIDE;
D O I
10.1152/jappl.1994.77.6.2519
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To determine whether nitric oxide (NO) synthase activity exists in rat skeletal muscle, media from incubated rat extensor digitorum longus muscle preparations were assayed for NO with a chemiluminescent detection system. Although small amounts of NO were detected in media alone, the addition of muscle increased NO concentration in the media by 30-fold. The release of NO into the media diminished over time. Either arginine (10(-6) M), sodium nitroprusside (10(-6) M), or prior electrical stimulation in vivo caused 50-200% increases (P < 0.05) in NO concentration. N-G-monomethyl-L-arginine monoacetate (10(-6) M), an NO synthase inhibitor, decreased both basal 2-deoxyglucose transport and NO efflux, indicating that NO may play a role in modulating skeletal muscle carbohydrate metabolism. These data indicate that NO is released from an incubated skeletal muscle preparation and presents the possibility that muscle-derived NO may play an important metabolic role.
引用
收藏
页码:2519 / 2521
页数:3
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