ENDOTHELIAL-CELLS ACTIVELY SYNTHESIZE AND SECRETE ADRENOMEDULLIN

被引:553
作者
SUGO, S
MINAMINO, N
KANGAWA, K
MIYAMOTO, K
KITAMURA, K
SAKATA, J
ETO, T
MATSUO, H
机构
[1] NATL CARDIOVASC CTR,RES INST,SUITA,OSAKA 565,JAPAN
[2] MIYAZAKI MED COLL,DEPT MED,MIYAZAKI 88916,JAPAN
关键词
D O I
10.1006/bbrc.1994.1827
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study demonstrates active production of adrenomedullin (AM) in cultured vascular endothelial cells (ECs). To identify the origin of plasma AM and its functional relationship to vascular smooth muscle cells (VSMCs), we checked production of AM in a series of tissues and cell lines and found immunoreactive (ir-) AM in culture media of rat, porcine, human and bovine ECs. Ir-AM was accumulated linearly for up to 48 hours in the culture medium of rat ECs, and the secretion rate of AM was almost comparable to that of endothelin-1. By gel filtration and reverse phase high performance liquid chromatography, ir-AM in the culture medium was shown to have chromatographic behavior indistinguishable from that of synthetic rat AM. By RNA blot analysis of rat tissue, the most highly positive band was detected in cultured ECs, at an intensity 20 to 40 fold higher than that in adrenal gland. Based on these data as well as the presence of AM specific receptor on VSMCs, AM secreted from ECs is deduced to act directly on VSMCs, regulating vascular tone. (C) 1994 Academic Press, Inc.
引用
收藏
页码:1160 / 1166
页数:7
相关论文
共 24 条
[1]   CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR [J].
BRAIN, SD ;
WILLIAMS, TJ ;
TIPPINS, JR ;
MORRIS, HR ;
MACINTYRE, I .
NATURE, 1985, 313 (5997) :54-56
[2]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3]   SPECIFIC RECEPTORS FOR ADRENOMEDULLIN IN CULTURED RAT VASCULAR SMOOTH-MUSCLE CELLS [J].
EGUCHI, S ;
HIRATA, Y ;
KANO, H ;
SATO, K ;
WATANABE, Y ;
WATANABE, TX ;
NAKAJIMA, K ;
SAKAKIBARA, S ;
MARUMO, F .
FEBS LETTERS, 1994, 340 (03) :226-230
[4]   CDNA CLONING AND EXPRESSION OF RAT-TISSUE FACTOR PATHWAY INHIBITOR (TFPI) [J].
ENJYOJI, K ;
EMI, M ;
MUKAI, T ;
KATO, H .
JOURNAL OF BIOCHEMISTRY, 1992, 111 (05) :681-687
[5]   NEUROGENIC VASODILATION AND RELEASE OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) FROM PERIVASCULAR NERVES IN THE RAT MESENTERIC-ARTERY [J].
FUJIMORI, A ;
SAITO, A ;
KIMURA, S ;
WATANABE, T ;
UCHIYAMA, Y ;
KAWASAKI, H ;
GOTO, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1391-1398
[6]   ENDOTHELIUM-DERIVED RELAXING AND CONTRACTING FACTORS [J].
FURCHGOTT, RF ;
VANHOUTTE, PM .
FASEB JOURNAL, 1989, 3 (09) :2007-2018
[7]  
GIRGIS SI, 1985, LANCET, V2, P14
[8]   DISTRIBUTION AND CHARACTERIZATION OF IMMUNOREACTIVE ADRENOMEDULLIN IN HUMAN TISSUE AND PLASMA [J].
ICHIKI, Y ;
KITAMURA, K ;
KANGAWA, K ;
KAWAMOTO, M ;
MATSUO, H ;
ETO, T .
FEBS LETTERS, 1994, 338 (01) :6-10
[9]   HEMODYNAMIC-EFFECTS OF A NOVEL HYPOTENSIVE PEPTIDE, HUMAN ADRENOMEDULLIN, IN RATS [J].
ISHIYAMA, Y ;
KITAMURA, K ;
ICHIKI, Y ;
NAKAMURA, S ;
KIDA, O ;
KANGAWA, K ;
ETO, T .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 241 (2-3) :271-273
[10]   ADRENOMEDULLIN STIMULATES CYCLIC-AMP FORMATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS [J].
ISHIZAKA, Y ;
ISHIZAKA, Y ;
TANAKA, M ;
KITAMURA, K ;
KANGAWA, K ;
MINAMINO, N ;
MATSUO, H ;
ETO, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 200 (01) :642-646