DIRECT INTRACEREBRAL GENE-TRANSFER OF AN ADENOVIRAL VECTOR EXPRESSING TYROSINE-HYDROXYLASE IN A RAT MODEL OF PARKINSONS-DISEASE

被引：141

作者：

HORELLOU, P

VIGNE, E

CASTEL, MN

BARNEOUD, P

COLIN, P

PERRICAUDET, M

DELAERE, P

MALLET, J

机构：

[1] CNRS,GENET MOLEC NEUROTRANSMISS & PROC DEGENERATIFS LA,C 9923,F-91198 GIF SUR YVETTE,FRANCE

[2] INST GUSTAVE ROUSSY,CNRS,URA 1301,PR2,F-94805 VILLEJUIF,FRANCE

[3] RHONE POULENC RORER,CTR RECH VITRY ALFORTVILLE,F-94403 VITRY,FRANCE

来源：

NEUROREPORT | 1994年 / 6卷 / 01期

关键词：

TYROSINE HYDROXYLASE; PARKINSONS DISEASE; RECOMBINANT ADENOVIRUS; STRIATUM; 6-HYDROXYDOPAMINE; INTRACEREBRAL INJECTION; APOMORPHINE-INDUCED ROTATION; IN VIVO GENE THERAPY;

D O I：

10.1097/00001756-199412300-00014

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

DIRECT intracerebral gene transfer to neural cells has been demonstrated with recombinant adenovirus encoding beta-galactosidase. To explore the potential of recombinant adenovirus for the therapy of neurological disease we constructed a recombinant adenovirus encoding tyrosine hydroxylase and optimized intracerebral injection to express the gene in the striatum of unilaterally denervated rats. These animals have dopamine depletion in their lesioned striatum, causing a rotation asymmetry induced by apomorphine. One and two weeks after intracerebral injection tl;is sensorimotor asymmetry was decreased by the adenovirus encoding tyrosine hydroxylase and not by a control adenovirus encoding beta-galactosidase. Histological analysis showed that tyrosine hydroxylase was preferentially expressed in astrocytes.

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页码：49 / 53

页数：5

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