BIOPOLYMER SYNTHESIS ON POLYPROPYLENE SUPPORTS .1. OLIGONUCLEOTIDES

被引：51

作者：

MATSON, RS

RAMPAL, JB

COASSIN, PJ

机构：

[1] Advanced Development Unit, Beck man Instruments, Fullerton, CA 92634

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 217卷 / 02期

关键词：

D O I：

10.1006/abio.1994.1123

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have modified polypropylene to serve as a new solid-phase support for oligonucleotide synthesis. The plastic is first surface aminated by exposure to an ammonia plasma generated by radiofrequency plasma discharge. The aminated polypropylene has been found to be useful as a support for the in situ synthesis of oligonucleotides from monomers. Furthermore, oligonucleotides synthesized on the surface of the plastic remain attached following deprotection and can be used directly for hybridization. (C) 1994 Academic Press, Inc.

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页码：306 / 310

页数：5

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