NEW PLANT BINARY VECTORS WITH SELECTABLE MARKERS LOCATED PROXIMAL TO THE LEFT T-DNA BORDER

被引：540

作者：

BECKER, D

KEMPER, E

SCHELL, J

MASTERSON, R

机构：

[1] Max-Planck-Institut für Züchtungsforschung, Köln 30, D-5000

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 20卷 / 06期

关键词：

AGROBACTERIUM-TUMEFACIENS; T-DNA; PLANT TRANSFORMATION; BINARY VECTORS; SELECTABLE MARKERS;

D O I：

10.1007/BF00028908

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.

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页码：1195 / 1197

页数：3

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