CDNA CLONING AND CHARACTERIZATION OF RET ACTIVATED IN A HUMAN PAPILLARY THYROID-CARCINOMA CELL-LINE

被引：103

作者：

ISHIZAKA, Y

USHIJIMA, T

SUGIMURA, T

NAGAO, M

机构：

[1] Carcinogenesis Division, National Cancer Center Research Institute 1-1, 104, Tsukiji-5, Chuo-ku, Tokyo, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92335-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We obtained activated ret cDNAs (retTPC) from a human papillary thyroid carcinoma cell line, TPC-1, and characterized its structure. The nucleotide sequence indicated that the recombination had occurred just upstream of the kinase domain of ret proto-oncogene and that the position, where the conserved sequence of ret proto-oncogene starts in retTPC transcripts, was exactly the same as that of ret-II which we have previously analyzed. Furthermore, a unique 13-glycine stretch, which is also present in a small subunit of the calcium dependent protease, calpain, was detected in the replaced sequence of retTPC. The aberrant tyrosine kinase activity induced by the rearrangement of ret proto-oncogene could be involved in the development of papillary thyroid carcinoma. © 1990.

引用

页码：402 / 408

页数：7

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