STUDIES ON HUMAN PORIN .13. THE TYPE-1 VDAC PORIN 31HL BIOTINYLATED AT THE PLASMALEMMA OF TRYPAN BLUE EXCLUDING HUMAN B-LYMPHOCYTES

被引：26

作者：

JAKOB, C ^{[1
]}

GOTZ, H ^{[1
]}

HELLMANN, T ^{[1
]}

HELLMANN, KP ^{[1
]}

REYMANN, S ^{[1
]}

FLORKE, H ^{[1
]}

THINNES, FP ^{[1
]}

HILSCHMANN, N ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL MED,IMMUNCHEM ABT,D-37075 GOTTINGEN,GERMANY

来源：

FEBS LETTERS | 1995年 / 368卷 / 01期

关键词：

PORIN; 31HL; VDAC; CHLORIDE CHANNELS; BIOTIN; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1016/0014-5793(95)00465-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC, Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues, Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells, Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane.

引用

页码：5 / 9

页数：5

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