STRUCTURE AND SEQUENCE OF THE RFB (O-ANTIGEN) GENE-CLUSTER OF SALMONELLA SEROVAR TYPHIMURIUM (STRAIN-LT2)

被引：340

作者：

JIANG, XM ^{[1
]}

NEAL, B ^{[1
]}

SANTIAGO, F ^{[1
]}

LEE, SJ ^{[1
]}

ROMANA, LK ^{[1
]}

REEVES, PR ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT MICROBIOL,SYDNEY,NSW 2006,AUSTRALIA

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 03期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00741.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rfb gene cluster of Salmonella LT2 has been cloned and sequenced. The genes rfbA, rfbB, rfbD, rfbF, rfbG, rfbK, rfbM and rfbP were located individually and the gene rfbL was located outside the cluster. Approximately 16 open reading frames were found in the region which is essential for the expression of O antigen. The gene products of rfbB and rfbG were found to have homology with the group of dehydrogenase and related enzymes described previously. Analysis of the G + C ratio of the rfb cluster extended the area of low-G + C composition previously found in the sequence of rfbJ to the whole rfb gene cluster. Three to five segments with discrete G + C contents and codon adaptation indices are present in the rfb region, indicating a heterogeneous origin of these segments. Potential promoters were found near the start of the rfb region, supporting the possibility that the rfb gene cluster is an operon.

引用

页码：695 / 713

页数：19

共 63 条

[1]

Bachmann B. J., 1987, ESCHERICHIA COLI SAL, V2, P807

[2] DOUBLE COS SITE VECTORS - SIMPLIFIED COSMID CLONING [J].