HYPOTONIC SOLUTION INCREASES THE SLOWLY ACTIVATING POTASSIUM CURRENT ISK EXPRESSED IN XENOPUS OOCYTES

被引：47

作者：

BUSCH, AE

VARNUM, M

ADELMAN, JP

NORTH, RA

机构：

[1] Vollum Institute, Oregon Health Sciences University, Portland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90661-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network. © 1992.

引用

页码：804 / 810

页数：7

共 21 条

[1]

BUSCH AE, 1992, IN PRESS SCIENCE

[2]

BUSCH AE, 1992, IN PRESS J PHYSL

[3] ACTIN-FILAMENTS REGULATE EPITHELIAL NA+ CHANNEL ACTIVITY [J].