SINGLE-MOLECULE DETECTION AND PHOTOCHEMISTRY ON A SURFACE USING NEAR-FIELD OPTICAL-EXCITATION

被引：199

作者：

AMBROSE, WP ^{[1
]}

GOODWIN, PM ^{[1
]}

MARTIN, JC ^{[1
]}

KELLER, RA ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545

来源：

PHYSICAL REVIEW LETTERS | 1994年 / 72卷 / 01期

关键词：

D O I：

10.1103/PhysRevLett.72.160

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We report the first photobleaching experiments of single molecules on a surface. Single molecules of rhodamine 6G are detected on a silica surface under ambient conditions using near-field scanning optical microscopy. Photobleaching of an individual molecule appears as an abrupt disappearance of fluorescence after seconds of constant excitation. Other reversible, upward and downward changes in fluorescence are observed, possibly due to reorientation of molecules on the surface.

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页码：160 / 163

页数：4

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