BICARBONATE-DEPENDENT CHANGES OF INTRACELLULAR SODIUM AND PH IN IDENTIFIED LEECH GLIAL-CELLS

被引:20
作者
DEITMER, JW
机构
[1] Abteilung für Allgemeine Zoologie, FB Biologie, Universität Kaiserslautern, Kaiserslautern, W-6750
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1992年 / 420卷 / 5-6期
关键词
BICARBONATE; INTRACELLULAR SODIUM; INTRACELLULAR PH; GLIAL CELLS; LEECH;
D O I
10.1007/BF00374637
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A new triple-barrelled ion-sensitive microelectrode was used to investigate the importance of bicarbonate for the regulation of intracellular Na+ and pH (Na(i) and pH(i), respectively) of neuropile glial cells in the central nervous system of the leech Hirudo medicinalis. Addition Of CO2/HCO3- produced an increase of the Na(i) activity and an intracellular alkalinization, indicating bicarbonate accumulation in the glial cells. Changes of external pH (from 7.4 to 7.0 and 7.8) produced large and rapid shifts of pH(i) and Na(i) and of the membrane potential in the presence, but not in the absence, of bicarbonate. Thus, acid/base transport and Na+ movements across the glial membrane into and out of the cells were accelerated severalfold in CO2/HCO3--buffered saline as compared to a CO2/HCO3--free, HEPES-buffered saline. The results suggest that the electrogenic, reversible, cotransport of Na+ and HCO3- in the glial cell membrane [3, 9] can produce significant changes in intraglial pH and Na activity, and can carry a significant fraction of the total Na+ flux across the cell membrane.
引用
收藏
页码:584 / 589
页数:6
相关论文
共 15 条
[1]   ELECTROGENIC NA+/HCO3- COTRANSPORT IN NEUROGLIA [J].
ASTION, ML ;
ORKAND, RK .
GLIA, 1988, 1 (05) :355-357
[2]   THE ELECTROGENIC NA/HCO3 COTRANSPORTER [J].
BORON, WF ;
BOULPAEP, EL .
KIDNEY INTERNATIONAL, 1989, 36 (03) :392-402
[3]   THE REGULATION OF INTRACELLULAR PH BY IDENTIFIED GLIAL-CELLS AND NEURONS IN THE CENTRAL-NERVOUS-SYSTEM OF THE LEECH [J].
DEITMER, JW ;
SCHLUE, WR .
JOURNAL OF PHYSIOLOGY-LONDON, 1987, 388 :261-283
[4]   MEMBRANE-POTENTIAL DEPENDENCE OF INTRACELLULAR PH REGULATION BY IDENTIFIED GLIAL-CELLS IN THE LEECH CENTRAL-NERVOUS-SYSTEM [J].
DEITMER, JW ;
SZATKOWSKI, M .
JOURNAL OF PHYSIOLOGY-LONDON, 1990, 421 :617-631
[5]   EVIDENCE FOR GLIAL CONTROL OF EXTRACELLULAR PH IN THE LEECH CENTRAL-NERVOUS-SYSTEM [J].
DEITMER, JW .
GLIA, 1992, 5 (01) :43-47
[6]   INTRACELLULAR ACIDOSIS OF IDENTIFIED LEECH NEURONS PRODUCED BY SUBSTITUTION OF EXTERNAL SODIUM [J].
DEITMER, JW ;
SCHLUE, WR .
BRAIN RESEARCH, 1988, 462 (02) :233-241
[7]  
DEITMER JW, 1981, J EXP BIOL, V91, P87
[8]   AN INWARDLY DIRECTED ELECTROGENIC SODIUM-BICARBONATE CO-TRANSPORT IN LEECH GLIAL-CELLS [J].
DEITMER, JW ;
SCHLUE, WR .
JOURNAL OF PHYSIOLOGY-LONDON, 1989, 411 :179-194
[9]   ELECTROGENIC SODIUM-DEPENDENT BICARBONATE SECRETION BY GLIAL-CELLS OF THE LEECH CENTRAL-NERVOUS-SYSTEM [J].
DEITMER, JW .
JOURNAL OF GENERAL PHYSIOLOGY, 1991, 98 (03) :637-655
[10]  
DEITMER JW, 1991, 19TH P GOTT NEUR C