DERIVATIZATION AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC DETECTION OF ANABOLIC-STEROID RESIDUES ISOLATED FROM EDIBLE MUSCLE TISSUES

被引：48

作者：

DAESELEIRE, E

DEGUESQUIERE, A

VANPETEGHEM, C

机构：

[1] Laboratory of Food Analysis, State University of Ghent, B-9000 Ghent

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80617-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed for the detection of anabolic steroid residues in edible muscle tissues. After enzymic digestion of the tissue and purification on disposable C18 solid-phase extraction columns, the extract was injected onto a C18 reversed-phase high-performance liquid chromatographic column. Three fractions or windows were collected, each containing specific analytes. After evaporation to dryness, the residues were subjected to a derivatization procedure which yielded suitable derivatives. After gas chromatographic-mass spectrometric analysis, both gas chromatographic retention data and mass spectral data were used for the detection and identification of nortestosterone, testosterone, estradiol, ethinylestradiol, trenbolone, methyltestosterone, chlormadinone acetate, medroxyprogesterone acetate and megestrol acetate.

引用

页码：673 / 679

页数：7

共 23 条

[1] APPLICATION OF A CAPILLARY GAS-CHROMATOGRAPHIC SELECTED-ION RECORDING MASS-SPECTROMETRIC TECHNIQUE TO THE ANALYSIS OF DIETHYLSTILBESTROL AND ITS PHOSPHORYLATED PRECURSORS IN PLASMA AND TISSUES [J].