INTRAHYPOTHALAMIC INFUSION OF INTERLEUKIN-1-BETA INCREASES THE RELEASE OF CORTICOTROPIN-RELEASING HORMONE (CRH-41) AND ADRENOCORTICOTROPIC HORMONE (ACTH) IN FREE-MOVING RATS BEARING A PUSH-PULL CANNULA IN THE MEDIAN-EMINENCE

In two simultaneous studies on unanesthetized rats implanted 1 week earlier with either an intracerebral (i.c.) cannula adjacent to the paraventricular nucleus of the hypothalamus and an intracarotid cannula, or the same i.c. cannula together with a push-pull cannula in the median eminence (ME), we explored the effect of i.c. infused interleukin-1β (IL 1β, 5 ng in 0.25 μl of vehicle within 2 min) on the release of corticotropin-releasing hormone (CRH) 41 and adrenocorticotropic hormone (ACTH). Intracerebral infusion of the vehicle alone had no significant effect on either the pulsatility or the level of CRH 41 release and only a short-lived minor effect on plasma ACTH, whereas i.c. IL 1β injection led to a significant and long lasting (1-2 h) rise in CRH 41 release peaking 3 times higher than the mean peaks of basal pulsatility (26.1 ± 3.5 pg/5 min vs 9.5 ± 0.7 pg/5 min), and in plasma ACTH culminating 15-20 times higher than basal levels. Simultaneously, body temperature was increased by 2.3 ± 0.3 °C. In another experiment, i.c.v. infusion of IL 1β produced a similar increase in plasma ACTH in rats whose catecholaminergic innervation to the hypothalamus had been obliterated by a bilateral injection of 6-hydroxydopamine into the ventral noradrenergic bundle, which appears to rule out modulation of this innervation in the stimulatory effect of IL 1β. The precise cellular site of action of IL 1β on CRH 41 secreting neurons and the physiological relevance of the study are discussed within the framework of functional interactions between the neuroendocrine and immune systems. © 1990.