PURIFIED GLUCOCORTICOID RECEPTORS BIND SELECTIVELY INVITRO TO A CLONED DNA FRAGMENT THAT MEDIATES A DELAYED SECONDARY RESPONSE TO GLUCOCORTICOIDS INVIVO

被引：18

作者：

HESS, P ^{[1
]}

MEENAKSHI, T ^{[1
]}

CHAN, GCK ^{[1
]}

CARLSTEDTDUKE, J ^{[1
]}

GUSTAFSSON, JA ^{[1
]}

PAYVAR, F ^{[1
]}

机构：

[1] KAROLINSKA HOSP,HUDDINGE UNIV HOSP F69,DEPT MED NUTR,S-14186 HUDDINGE,SWEDEN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 07期

关键词：

Delayed secondary glucocorticoid response element; Late or indirect responses; Specific protein-DNA interactions; α[!sub]2u[!/sub]-globulin gene;

D O I：

10.1073/pnas.87.7.2564

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.

引用

页码：2564 / 2568

页数：5

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