PROCEDURE FOR ENZYMATIC-SYNTHESIS AND ISOLATION OF RADIOACTIVE LONG-CHAIN ACYL-COA ESTERS

被引:23
作者
BANIS, RJ [1 ]
ROBERTS, CS [1 ]
STOKES, GB [1 ]
TOVE, SB [1 ]
机构
[1] N CAROLINA STATE UNIV, DEPT BIOCHEM, RALEIGH, NC 27607 USA
关键词
D O I
10.1016/0003-2697(76)90135-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method is described that yields pure long chain fatty acyl-CoA esters. It utilizes a partially purified long chain acyl-CoA synthetase that can be obtained from various sources. Product isolation involves butanol extraction and TLC. It is applicable to a wide range of fatty acids and is particularly suitable in preparing radioactive acyl-CoA esters.
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页码:1 / 8
页数:8
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