EFFECTS OF TISSUE ABSORBENCY ON NAD(P)H AND INDO-1 FLUORESCENCE FROM PERFUSED RABBIT HEARTS

被引：45

作者：

FRALIX, TA

HEINEMAN, FW

BALABAN, RS

机构：

[1] Laboratory of Cardiac Energetics, NIH, Bethesda, MD 20892, Bldg 1

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

A23187; Calcium; Cytochrome; Hypoxia; Inner filter; Myoglobin; NADH; Optical spectroscopy; Spectrofluorometry;

D O I：

10.1016/0014-5793(90)80212-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue 'inner filter' effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 μm due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. Several potential methods of correcting for these tissue inner filter effects are discussed. © 1990.

引用

页码：287 / 292

页数：6

共 16 条

[1] SPECTROPHOTOMETRIC MONITORING OF O-2 DELIVERY TO THE EXPOSED RAT-KIDNEY [J].