A DEFINED SYSTEM FOR IN-VITRO LAMBDA-DNA PACKAGING

被引：38

作者：

HWANG, Y ^{[1
]}

FEISS, M ^{[1
]}

机构：

[1] UNIV IOWA,DEPT MICROBIOL,IOWA CITY,IA 52242

来源：

VIROLOGY | 1995年 / 211卷 / 02期

关键词：

D O I：

10.1006/viro.1995.1419

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We constructed a defined in vitro system for packaging lambda DNA which is composed of purified proheads, the noncapsid proteins terminase and gpFl, and the Escherichia coli DNA binding/bending protein IHF. The defined packaging system: (i) is free from endogenous ATP, DNAs, and DNases and (ii) packages 30% of the input mature lambda DNA efficiently. In this defined packaging system, IHF and gpFl make modest contributions to packaging efficiency. The defined packaging reactions showed that DNA packaging gave a linear response to the concentration of mature lambda DNA and terminase. DNA packaging showed a sigmoidal relationship with respect to the concentration of ATP and proheads. (C) 1995 Academic Press, Inc.

引用

页码：367 / 376

页数：10

共 57 条

[1]

ARBER W, 1983, LAMBDA, V2, P433

[2]

BAZINET C, 1985, ANNU REV MICROBIOL, V39, P109

[3] EARLY EVENTS IN INVITRO PACKAGING OF BACTERIOPHAGE-LAMBDA DNA [J].