PHORBOL ESTER INDUCES DESENSITIZATION OF PTH-STIMULATED CYCLIC-AMP PRODUCTION BY DECREASING THE PTH RECEPTOR-BINDING IN UMR-106 CELLS

被引：7

作者：

IKEDA, K

SUGIMOTO, T

FUKASE, M

FUJITA, T

机构：

[1] Third Division, Department of Medicine Kobe University School of Medicine, Kobe

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)80250-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pretreatment of UMR-106 cells(rat osteoblast like osteosarcoma cell line) with the protein kinase C(PK-C) activating phorbol ester, phorbol 12-myristate 13-acetate (PMA) results in a time dependent(1-12h) desensitization of PTH-stimulated cAMP production. Compared to controls, PMA-treated cells showed 50% decrease of PTH-stimulated cAMP production. PK-C inhibitor, H-7 significantly blocked this PMA-induced desensitization. PTH receptor binding, assessed with 125I-[Nle8,Nle18, Tyr34]PTH-(1-34) as radioligand, was decreased by about 20% in PMA-treated cells. H-7 treatment completely restored receptor binding in PMA-treated cells. These data suggest that PK-C might act directly on PTH receptor which is coupling to adenylate cyclase, and induce desensitization. © 1991 Academic Press, Inc.

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页码：764 / 768

页数：5

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