NOVEL INTEGRIN ALPHA-SUBUNIT AND BETA-SUBUNIT CDNAS IDENTIFIED IN AIRWAY EPITHELIAL-CELLS AND LUNG LEUKOCYTES USING THE POLYMERASE CHAIN-REACTION

The integrins are a large group of cell surface glycoproteins that mediate cell-matrix and cell-cell adhesive interactions. Integrins play a role in normal lung development, in host defense against pulmonary infection, and in the pathogenesis of the adult respiratory distress syndrome. Integrins are heterodimers consisting of one alpha-subunit and one beta-subunit. We identified consensus sequences within integrin subunits and used oligonucleotide primers based on these sequences to amplify cDNA by the polymerase chain reaction (PCR). We previously reported the use of this homology PCR technique for the identification of one novel integrin beta-subunit, beta-6, from guinea pig airway epithelial cells. Here we demonstrate that primers based on alpha-subunit consensus sequences can also be used for homology PCR. We have used the alpha and beta-subunit primers to amplify and clone a large variety of integrin partial cDNAs from several cell types and species. Comparison of the deduced amino acid sequences reveals a high degree of cross-species conservation (86 to 98% identity). One alpha-subunit (identified in guinea pig airway epithelial cells) and one beta-subunit (identified in rabbit leukocytes obtained by bronchoalveolar lavage and in human and mouse leukocyte cell lines) have novel sequences that are related to but clearly distinct from all previously reported integrin sequences (24 to 61% identity). These novel cDNAs are very likely to encode previously unsequenced integrin subunit proteins that are expressed in the lung. Homology PCR is a powerful technique for the identification of known and novel integrin alpha and beta-subunit cDNAs in cells from the lung and other organs.