USING 2-DIMENSIONAL GEL-ELECTROPHORESIS TO STUDY IMMUNE-RESPONSE AGAINST INTRACELLULAR BACTERIAL-INFECTION

被引:5
作者
KOVAROVA, H
STULIK, J
MACELA, A
LEFKOVITS, I
SKRABKOVA, Z
机构
[1] PURKYNE MED ACAD, HRADEC KRALOVE, CZECHOSLOVAKIA
[2] BASEL INST IMMUNOL, CH-4005 BASEL, SWITZERLAND
关键词
D O I
10.1002/elps.11501301160
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Principal component analysis was applied to two-dimensional (2-D) gel electrophoresis patterns, obtained in various phases of infection. Untreated controls could be satisfactorily differentiated from patterns after infection on days 3 and 7 whereas day 10 of infection was grouped with the controls. Comparison of host cellular protein patterns could help to classify in vivo developing infection without requiring any so-called immune marker functions. Immunoaffinity separation of infected cells treated with detergent, followed by 2-D electrophoresis of negative as well as positive eluates, did not reveal radiolabeled bacterial protein antigens.
引用
收藏
页码:741 / 742
页数:2
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