BINDING OF GAP TO ACTIVATED PDGF RECEPTORS

被引：363

作者：

KAZLAUSKAS, A ^{[1
]}

ELLIS, C ^{[1
]}

PAWSON, T ^{[1
]}

COOPER, JA ^{[1
]}

机构：

[1] MT SINAI HOSP, SAMUEL LUNENFELD RES INST, DIV MOLEC & DEV BIOL, TORONTO M5G 1X5, ONTARIO, CANADA

来源：

SCIENCE | 1990年 / 247卷 / 4950期

关键词：

D O I：

10.1126/science.2157284

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ras proto-oncogene products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused approximately one-tenth of the total GAP molecules to complex with the receptor. Studies with mutant PDGF receptors showed that maximum association required both receptor kinase activity and phosphorylatable tyrosine residues at both the identified sites of receptor autophosphorylation.

引用

页码：1578 / 1581

页数：4

共 32 条

[1] GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN (GAP) INTERACTS WITH THE P21-RAS EFFECTOR BINDING DOMAIN [J].

ADARI, H ;

LOWY, DR ;

WILLUMSEN, BM ;

DER, CJ ;

MCCORMICK, F .

SCIENCE, 1988, 240 (4851) :518-520

[2] GENETIC-ANALYSIS OF MAMMALIAN GAP EXPRESSED IN YEAST [J].

BALLESTER, R ;

MICHAELI, T ;

FERGUSON, K ;

XU, HP ;

MCCORMICK, F ;

WIGLER, M .

CELL, 1989, 59 (04) :681-686

[3] RAS GENES [J].

BARBACID, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827

[4] THE CYTOPLASMIC PROTEIN GAP IS IMPLICATED AS THE TARGET FOR REGULATION BY THE RAS GENE-PRODUCT [J].

CALES, C ;

HANCOCK, JF ;

MARSHALL, CJ ;

HALL, A .

NATURE, 1988, 332 (6164) :548-551

[5] CDNA CLONING AND EXPRESSION OF A HUMAN PLATELET-DERIVED GROWTH-FACTOR (PDGF) RECEPTOR SPECIFIC FOR B-CHAIN-CONTAINING PDGF MOLECULES [J].

CLAESSONWELSH, L ;

ERIKSSON, A ;

MOREN, A ;

SEVERINSSON, L ;

EK, B ;

OSTMAN, A ;

BETSHOLTZ, C ;

HELDIN, CH .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (08) :3476-3486

[6]

COUGHLIN SR, 1989, SCIENCE, V243, P1191

[7] PHOSPHORYLATION OF GAP AND GAP-ASSOCIATED PROTEINS BY TRANSFORMING AND MITOGENIC TYROSINE KINASES [J].

ELLIS, C ;

MORAN, M ;

MCCORMICK, F ;

PAWSON, T .

NATURE, 1990, 343 (6256) :377-381

[8]

FRACKELTON AR, 1984, J BIOL CHEM, V259, P7909

[9] G-PROTEINS CONTROL DIVERSE PATHWAYS OF TRANSMEMBRANE SIGNALING [J].

FREISSMUTH, M ;

CASEY, PJ ;

GILMAN, AG .

FASEB JOURNAL, 1989, 3 (10) :2125-2131

[10] XENOPUS OOCYTE GERMINAL-VESICLE BREAKDOWN INDUCED BY [VAL12]RAS IS INHIBITED BY A CYTOSOL-LOCALIZED RAS MUTANT [J].

GIBBS, JB ;

SCHABER, MD ;

SCHOFIELD, TL ;

SCOLNICK, EM ;

SIGAL, IS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6630-6634

← 1 2 3 4 →