CLONING AND EXPRESSION OF APICAL MEMBRANE WATER CHANNEL OF RAT-KIDNEY COLLECTING TUBULE

被引：801

作者：

FUSHIMI, K ^{[1
]}

UCHIDA, S ^{[1
]}

HARA, Y ^{[1
]}

HIRATA, Y ^{[1
]}

MARUMO, F ^{[1
]}

SASAKI, S ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV,DEPT BIOCHEM,TOKYO 113,JAPAN

来源：

NATURE | 1993年 / 361卷 / 6412期

关键词：

D O I：

10.1038/361549a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

CONCENTRATING urine is mandatory for most mammals to prevent water loss from the body. Concentrated urine is produced in response to vasopressin by the transepithelial recovery of water from the lumen of the kidney collecting tubule through highly water-permeable membranes1,2. In this nephron segment, vasopressin regulates water permeability by endo- and exocytosis of water channels from or to the apical membrane3,4. CHIP28 is a water channel in red blood cells and the kidney proximal tubule5, but it is not expressed in the collecting tubule6. Here we report the cloning of the complementary DNA for WCH-CD, a water channel of the apical membrane of the kidney collecting tubule. WCH-CD is 42% identical in amino-acid sequence to CHIP28. WCH-CD transcripts are detected only in the collecting tubule of the kidney. Immunohistochemically, WCH-CD is localized to the apical region of the kidney collecting tubule cells. Expression of WCH-CD in Xenopus oocytes markedly increases osmotic water permeability. The functional expression and the limited localization of WCH-CD to the apical region of the kidney collecting tubule suggest that WCH-CD is the vasopressin-regulated water channel.

引用

页码：549 / 552

页数：4

共 24 条

[1] THE USE OF XENOPUS OOCYTES FOR THE STUDY OF ION CHANNELS [J].