DIRECT NONRADIOACTIVE INSITU HYBRIDIZATION OF SOMATIC-CELL HYBRID DNA TO HUMAN LYMPHOCYTE CHROMOSOMES

被引：58

作者：

KIEVITS, T

DEVILEE, P

WIEGANT, J

WAPENAAR, MC

CORNELISSE, CJ

VANOMMEN, GJB

PEARSON, PL

机构：

[1] UNIV LEIDEN,DEPT CYTOCHEM & CYTOMET,2333 LEIDEN,NETHERLANDS

[2] UNIV LEIDEN,DEPT PATHOL,2333 LEIDEN,NETHERLANDS

来源：

CYTOMETRY | 1990年 / 11卷 / 01期

关键词：

chromosome painting; Competitive in situ hybridization; hybrid characterization;

D O I：

10.1002/cyto.990110112

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotinylated DNA from various human–rodent hybrids was hybridized to human lymphocyte spreads after preannealing of the repeated sequences with sonicated total human DNA. Fluorescent labeling was achieved by successive treatments with fluorescein‐labeled avidin and biotinylated antiavidin antibody. The use of labeled total DNA from hybrids with known chromosome composition permits the fluorescent staining‐(“painting”) of specific chromosomes, or parts thereof, in human lymphocyte metaphases. Alternatively, the human chromosome content of cell hybrids with unknown chromosome composition is directly assessed from the labeling pattern of human lymphocyte spreads using the total hybrid DNA as probe. Copyright © 1990 Wiley‐Liss, Inc.

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页码：105 / 109

页数：5

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