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CDNA CLONING AND EXPRESSION OF A SOLUBLE EPOXIDE HYDROLASE FROM HUMAN LIVER

被引：144
作者：
BEETHAM, JK
TIAN, TG
HAMMOCK, BD
机构：
[1] UNIV CALIF DAVIS,DEPT ENTOMOL & ENVIRONM TOXICOL,DAVIS,CA 95616
[2] UNIV CALIF DAVIS,DEPT BIOCHEM & BIOPHYS,DAVIS,CA 95616
[3] UNIV CALIF DAVIS,DEPT PLANT PATHOL,DAVIS,CA 95616
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1993年 / 305卷 / 01期
关键词：
D O I：
10.1006/abbi.1993.1411
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the cloning and expression of a cDNA that encodes a soluble epoxide hydrolase from human liver. The 2101-base clone predicts a 554-residue protein (Mr 62,640) with an apparently imperfect peroxisomal targeting signal of Ser-Lys-Met at the carboxy terminus. The cDNA was expressed in the baculovirus system in the Spodoptera frugiperda 21 cell line. The recombinant protein was similar to soluble epoxide hydrolase isolated from human liver in terms of molecular weight, hydrolytic activity, inhibition, and immunoreactivity. © 1993 Academic Press, Inc.
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页码：197 / 201
页数：5
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