COMPARISON OF MRC-5 AND HFF CELLS FOR THE IDENTIFICATION OF CYTOMEGALOVIRUS IN CENTRIFUGATION CULTURE

被引：19

作者：

GLEAVES, CA

MEYERS, JD

机构：

[1] FRED HUTCHINSON CANC RES CTR, PROGRAM INFECT DIS, 1124 COLUMBIA ST, SEATTLE, WA 98104 USA

[2] FRED HUTCHINSON CANC RES CTR, DIAGNOST VIROL LAB, SEATTLE, WA 98104 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1987年 / 6卷 / 02期

关键词：

D O I：

10.1016/0732-8893(87)90105-2

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Detection of cytomegalovirus (CMV) by centrifugation culture was compared in MRC-5 and HFF cells. At 24 hr 47 of 130 specimens (36%) were positive for CMV, 45 in MRC-5, and 35 in HFF cells. At 48 hr 45 specimens were positive in both MRC-5 and HFF cells. MRC-5 cells appear to be superior for the detection of CMV at 24 hr in centrifugation culture; however, both lines are comparable at 48 hr.

引用

收藏

页码：179 / 182

页数：4

相关论文

共 11 条

[1] RAPID DETECTION OF HUMAN CYTOMEGALO-VIRUS IN THE URINE OF HUMANS [J].

ALPERT, G ;

MAZERON, MC ;

COLIMON, R ;

PLOTKIN, S .

JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) :631-633

[2]

FRIEDMAN HM, 1978, J CLIN MICROBIOL, V7, P368

[3] COMPARISON OF STANDARD TUBE AND SHELL VIAL CELL-CULTURE TECHNIQUES FOR THE DETECTION OF CYTOMEGALO-VIRUS IN CLINICAL SPECIMENS [J].

GLEAVES, CA ;

SMITH, TF ;

SHUSTER, EA ;

PEARSON, GR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (02) :217-221

[4] MONOCLONAL-ANTIBODIES TO CYTOMEGALOVIRUS - RAPID IDENTIFICATION OF CLINICAL ISOLATES AND PRELIMINARY USE IN DIAGNOSIS OF CYTOMEGALOVIRUS PNEUMONIA [J].

GOLDSTEIN, LC ;

MCDOUGALL, J ;

HACKMAN, R ;

MEYERS, JD ;

THOMAS, ED ;

NOWINSKI, RC .

INFECTION AND IMMUNITY, 1982, 38 (01) :273-281

[5] PRACTICAL PROTOCOL FOR CYTOMEGALOVIRUS ISOLATION - USE OF MRC-5 CELL MONOLAYERS INCUBATED FOR 2 WEEKS [J].

GREGORY, WW ;

MENEGUS, MA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 17 (04) :605-609

[6] OPTIMAL METHOD FOR RECOVERY OF CYTOMEGALOVIRUS FROM URINE OF RENAL-TRANSPLANT PATIENTS [J].

LEE, MS ;

BALFOUR, HH .

TRANSPLANTATION, 1977, 24 (03) :228-230

[7]

MUENCH R, 1985, ANNU M AM SOC MICROB, P285

[8]

REYNOLDS DW, 1979, DIAGNOSTIC PROCEDURE, P399

[9] MONOCLONAL-ANTIBODY FOR RAPID LABORATORY DETECTION OF CYTOMEGALO-VIRUS INFECTIONS - CHARACTERIZATION AND DIAGNOSTIC APPLICATION [J].

SHUSTER, EA ;

BENEKE, JS ;

TEGTMEIER, GE ;

PEARSON, GR ;

GLEAVES, CA ;

WOLD, AD ;

SMITH, TF .

MAYO CLINIC PROCEEDINGS, 1985, 60 (09) :577-585

[10] COMPARATIVE-STUDY OF DIAGNOSTIC PROCEDURES FOR CONGENITAL CYTOMEGALOVIRUS-INFECTION [J].

STAGNO, S ;

PASS, RF ;

REYNOLDS, DW ;

MOORE, MA ;

NAHMIAS, AJ ;

ALFORD, CA .

PEDIATRICS, 1980, 65 (02) :251-257