RELATIONSHIP BETWEEN HNRNA+-POLY-(A) AND MESSENGER-RNA+-POLY-(A) IN NON-DIVIDING HUMAN LYMPHOCYTES - EVIDENCE FOR DISTINCT SYNTHETIC PATHWAYS FOR MESSENGER-RNA PRECURSOR-HN-RNA AND NONPRECURSOR-HN-RNA

被引：14

作者：

BERGER, SL

COOPER, HL

机构：

[1] Section on Cellular and Molecular Physiology, Laboratory of Pathophysiology, National Institutes of Health, Bethesda

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 517卷 / 01期

关键词：

D O I：

10.1016/0005-2787(78)90036-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The processing of hnRNA+-poly(A) to mRNA+-poly(A) has been studied in resting lymphocytes from human peripheral blood. In pulse-chase experiments, two types of hnRNA+-poly(A) have been distinguished: the first is labeled predominantly with exogenous radioactive precursors supplied during the pulse, and the second incorporates primarily scavenged labeled precursors made available during a chase incubation. When the disappearance of both types of hnRNA+-poly(A) was quantitatively compared with the appearance of stable and labile mRNA+-poly(A), only 10% of the anticipated cytoplasmic material was actually obtained. Statistically, 90% of the poly(A)-bearing hnRNA molecules processed were degraded. The two types of hnRNA+-poly(A) were found to be functionally different. Pulse-labeled material was processed to poly(A)-bearing mRNA; chase-labeled" molecules did not leave the nucleus and never served as precursors for cytoplasmic mRNA. The data fit a model in which there are distinct pathways for mRNA precursor- and nonprecursor-hnRNA+-poly(A). © 1978."

引用

页码：84 / 98

页数：15

共 37 条

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