QUANTIFICATION OF FORMAZANS IN TISSUE-SECTIONS BY MICRODENSITOMETRY .3. EFFECT OF OBJECTIVE POWER AND SCANNING SPOT SIZE

被引：15

作者：

ALTMAN, FP ^{[1
]}

机构：

[1] INST ORTHOPAEDICS, PROF RES UNIT, STANMORE, MIDDLESEX, ENGLAND

来源：

HISTOCHEMICAL JOURNAL | 1976年 / 8卷 / 05期

关键词：

D O I：

10.1007/BF01003840

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The formazans derived from neotetrazolium chloride (NT) and 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride (BPST) can be measured by microdensitometry of tissue sections [mouse liver] with a wide range of scanning spot sizes, without any significant effect on the recorded extinctions. The spot sizes tested ranged from 0.20-16 .mu.m. Thus when large fields have to be measured, it is possible to use low-power objectives and still obtain valid results.

引用

页码：507 / 511

页数：5

共 5 条

[1]

ALTMAN FP, 1976, HISTOCHEM J, V8

[2] STUDIES ON REDUCTION OF TETRAZOLIUM SALTS .2. MEASUREMENT OF HALF REDUCED AND FULLY REDUCED FORMAZANS OF NEOTETRAZOLIUM CHLORIDE IN TISSUE SECTIONS [J].

BUTCHER, RG ;

ALTMAN, FP .

HISTOCHEMIE, 1973, 37 (04) :351-363

[3] ASPECTS OF SCANNING MICRODENSITOMETRY .2. SPOT SIZE, FOCUS AND RESOLUTION [J].

GOLDSTEIN, DJ .

JOURNAL OF MICROSCOPY, 1971, 93 (FEB) :15-+

[4]

GOLDSTEIN DJ, 1973, ENCY MICROSCOPY MICR, P531

[5] NONOSMIOPHILIC TETRAZOLIUM SALTS THAT YIELD OSMIOPHILIC, LIPOPHOBIC FORMAZANS FOR ULTRASTRUCTURAL LOCALIZATION OF DEHYDROGENASE-ACTIVITY [J].

KALINA, M ;

SELIGMAN, AM ;

HOSHINO, Y ;

PLAPINGER, RE .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1972, 20 (09) :685-+

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