TRANSFORMING GROWTH FACTOR-BETA-1 INHIBITION OF P34CDC2 PHOSPHORYLATION AND HISTONE-H1 KINASE-ACTIVITY IS ASSOCIATED WITH G1/S-PHASE GROWTH ARREST

被引：220

作者：

HOWE, PH

DRAETTA, G

LEOF, EB

机构：

[1] VANDERBILT UNIV, DEPT CELL BIOL, NASHVILLE, TN 37232 USA

[2] EUROPEAN MOLEC BIOL LAB, W-6900 HEIDELBERG, GERMANY

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 03期

关键词：

D O I：

10.1128/MCB.11.3.1185

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor beta-1 (TGF-beta-1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGF-beta-1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGF-beta-1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-beta-D-ribofurano-sylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGF-beta-1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.

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页码：1185 / 1194

页数：10

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