HIGHLY INDUCIBLE SYNTHESIS OF HETEROLOGOUS PROTEINS IN EPITHELIAL-CELLS CARRYING A GLUCOCORTICOID-RESPONSIVE VECTOR

被引：58

作者：

HIRT, RP

POULAINGODEFROY, O

BILLOTTE, J

KRAEHENBUHL, JP

FASEL, N

机构：

[1] UNIV LAUSANNE,INST BIOCHEM,CHEM BOVERESSES 155,CH-1066 EPALINGES,SWITZERLAND

[2] SWISS INST EXPTL CANC RES,CH-1066 EPALINGES,SWITZERLAND

来源：

GENE | 1992年 / 111卷 / 02期

关键词：

RECOMBINANT DNA; MAMMARY TUMOR VIRUS; LONG TERMINAL REPEAT;

D O I：

10.1016/0378-1119(92)90687-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.

引用

页码：199 / 206

页数：8

共 30 条

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