LARGE-SCALE CDNA SEQUENCING FOR ANALYSIS OF QUANTITATIVE AND QUALITATIVE ASPECTS OF GENE-EXPRESSION

被引：454

作者：

OKUBO, K ^{[1
]}

HORI, N ^{[1
]}

MATOBA, R ^{[1
]}

NIIYAMA, T ^{[1
]}

FUKUSHIMA, A ^{[1
]}

KOJIMA, Y ^{[1
]}

MATSUBARA, K ^{[1
]}

机构：

[1] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,1-3 YAMADA OKA,SUITA,OSAKA 565,JAPAN

来源：

NATURE GENETICS | 1992年 / 2卷 / 03期

关键词：

D O I：

10.1038/ng1192-173

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2, that is a non-biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein-synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non-abundant, consisting of about 6,600 genes.

引用

页码：173 / 179

页数：7

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